Thériault L, Pagé M
Clin Chem. 1977 Nov;23(11):2142-4.
We describe an enzyme immunoassay for human serum ferritin in which antibody adsorbed on polystyrene tubes is used. Adsorbed gamma-globulins against human ferritin were first allowed to react with ferritin and a second antiferritin antibody, labeled with alkaline phosphatase, was added. The amount of bound enzyme/antibody conjugate was proportional to the ferritin titer in the assay. This method offers stable reagents that can be kept for many months at 4 degrees C. The average values for ferritin in normal men and women were, respectively, 58 and 43 microgram/liter. The lowest detectable concentration was 5 microgram/liter.
我们描述了一种用于检测人血清铁蛋白的酶免疫测定法，该方法使用吸附在聚苯乙烯管上的抗体。首先使吸附的抗人铁蛋白γ球蛋白与铁蛋白反应，然后加入用碱性磷酸酶标记的第二抗铁蛋白抗体。结合的酶/抗体缀合物的量与测定中铁蛋白的效价成正比。该方法提供了稳定的试剂，可在4℃下保存数月。正常男性和女性铁蛋白的平均值分别为58和43微克/升。最低可检测浓度为5微克/升。
