梅毒螺旋体的丙酮酸氧化作用
Pyruvate oxidation by Treponema pallidum.
作者信息
Barbieri J T, Cox C D
出版信息
Infect Immun. 1979 Jul;25(1):157-63. doi: 10.1128/iai.25.1.157-163.1979.
Abstract
Cell-free extracts of Treponema pallidum catalyzed the decarboxylation of pyruvate. This activity was suppressed at low O2 tensions and appeared to be coenzyme A independent. Pyruvate decarboxylation was inorganic phosphate dependent, and evidence suggested that acetyl phosphate was a product. Oxygen was consumed, and data indicated that H2O2 was produced. These results indicated that the overall oxidation of pyruvate was: pyruvate + O2 + inorganic phosphate leads to CO2 + acetyl phosphate + H2O2. Phosphotransacetylase and acetate kinase activities were also observed in the cell-free extracts and could catalyze formation of acetyl coenzyme A and adenosine 5'-triphosphate, respectively, from acetyl phosphate.
摘要
梅毒螺旋体的无细胞提取物催化丙酮酸的脱羧反应。这种活性在低氧张力下受到抑制，并且似乎不依赖辅酶A。丙酮酸脱羧反应依赖无机磷酸盐，有证据表明乙酰磷酸是产物。消耗了氧气，数据表明产生了过氧化氢。这些结果表明丙酮酸的总体氧化反应为：丙酮酸+氧气+无机磷酸盐→二氧化碳+乙酰磷酸+过氧化氢。在无细胞提取物中还观察到磷酸转乙酰酶和乙酸激酶活性，它们分别可以催化由乙酰磷酸形成乙酰辅酶A和腺苷5'-三磷酸。
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