特定脱氧寡核苷酸诱导大肠杆菌K-12中的原噬菌体（phi 80）
Prophage (phi 80) induction in Escherichia coli K-12 by specific deoxyoligonucleotides.
作者信息
Irbe R M, Morin L M, Oishi M
出版信息
Proc Natl Acad Sci U S A. 1981 Jan;78(1):138-42. doi: 10.1073/pnas.78.1.138.
Abstract
A cell preparation that is permeable to proteins and oligonucleotides yet produces infectious phage particles after induction treatments was obtained by plasmolysis of Escherichia coli cells lysogenic for phi 80. When the permeabilized cells were exposed to specific oligo(deoxynucleotides), prophage (phi 80) was induced during further incubation. Of the dinucleotides tested, only d(A-G), d(G-G), and d(I-G) induced prophage. The essential base sequence of the deoxydinucleotides for the induction was determined to be deoxy(purine-G). Among oligo(deoxynucleotides) with unique base composition examined, only oligo(deoxyguanylates) exhibited the inducing activity. Although this specific oligo(deoxynucleotide)-triggered induction occurred in recB- cells, the induction was not detected in recA- cells or in the cells lysogenic for induction-negative phi 80(ind-). Possible biological significance of the oligo(deoxynucleotide)-triggered prophage induction is discussed.
摘要
通过对溶源化有φ80的大肠杆菌细胞进行质壁分离，获得了一种对蛋白质和寡核苷酸具有通透性、但在诱导处理后能产生感染性噬菌体颗粒的细胞制剂。当使通透化细胞暴露于特定的寡聚（脱氧核苷酸）时，在进一步孵育过程中前噬菌体（φ80）被诱导。在所测试的二核苷酸中，只有d(A-G)、d(G-G)和d(I-G)能诱导前噬菌体。确定诱导所需的脱氧二核苷酸的基本碱基序列为脱氧（嘌呤-G）。在所检测的具有独特碱基组成的寡聚（脱氧核苷酸）中，只有寡聚（脱氧鸟苷酸）表现出诱导活性。尽管这种特定的寡聚（脱氧核苷酸）触发的诱导发生在recB-细胞中，但在recA-细胞或溶源化有诱导阴性的φ80(ind-)的细胞中未检测到诱导现象。文中讨论了寡聚（脱氧核苷酸）触发的前噬菌体诱导可能的生物学意义。
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