通透细胞系统中的原噬菌体诱导：脱氧核糖核酸酶诱导及recBC - 脱氧核糖核酸酶的作用
Prophage induction in a permeabilized cell system: induction by deoxyribonucleases and the role of recBC-deoxyribonuclease.
作者信息
Irbe R M, Oishi M
出版信息
J Bacteriol. 1980 Dec;144(3):1061-7. doi: 10.1128/jb.144.3.1061-1067.1980.
Abstract
Permeabilized cells able to induce prophage were obtained by plasmolysis and preincubation of the cells in a reaction mixture which allows protein synthesis. These cells became permeable to low-molecular-weight proteins and oligonucleotides. We found that deoxyribonucleases (pancreatic deoxyribonuclease and micrococcal nuclease) triggered prophage (phi 80) induction. This deoxyribonuclease-triggered induction was completely dependent upon the presence of functional recBC genes in the lysogen, regardless of the recombination proficiency determined by recBC and sbcB genes. The possible role of recBC-deoxyribonuclease in prophage induction and recombination is discussed.
摘要
通过质壁分离以及将细胞在允许蛋白质合成的反应混合物中进行预孵育，获得了能够诱导前噬菌体的透化细胞。这些细胞对低分子量蛋白质和寡核苷酸具有通透性。我们发现脱氧核糖核酸酶（胰脱氧核糖核酸酶和微球菌核酸酶）可触发前噬菌体（φ80）的诱导。这种由脱氧核糖核酸酶触发的诱导完全依赖于溶原菌中功能性recBC基因的存在，而与recBC和sbcB基因所决定的重组能力无关。文中讨论了recBC - 脱氧核糖核酸酶在噬菌体诱导和重组中的可能作用。
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