Oishi M, Smith C L
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3569-73. doi: 10.1073/pnas.75.8.3569.
UV light causes inactivation of phage (phi80) repressor molecules in a plasmolyzed, permeable cell preparation of Escherichia coli. Induction without UV irradiation occurs when the permeable cells are incubated in the presence of four deoxyribonucleoside triphosphates and ATP. The induction triggered by dNTP's requires a functional recBC gene product and is associated with degradation of the DNA replication fork. The role of recBC DNase in the induction of prophage and SOS functions in general is discussed.
紫外线可使大肠杆菌经质壁分离的可渗透细胞制剂中的噬菌体(φ80)阻遏物分子失活。当可渗透细胞在四种脱氧核糖核苷三磷酸和ATP存在的情况下孵育时,会发生无紫外线照射的诱导。由脱氧核糖核苷三磷酸引发的诱导需要功能性的recBC基因产物,并且与DNA复制叉的降解有关。本文讨论了recBC核酸酶在噬菌体诱导及一般SOS功能中的作用。