Pruul H, Wetherall B L, McDonald P J
Antimicrob Agents Chemother. 1981 Jun;19(6):945-51. doi: 10.1128/AAC.19.6.945.
The effect of brief exposure to chloramphenicol of a pathogenic strain of Escherichia coli on susceptibility to normal human leukocytes was examined. Leukocytes killed chloramphenicol-pretreated E. coli more efficiently than they did untreated controls. Phagocytosis of pretreated bacteria, as measured by the uptake of radiolabeled bacteria and by direct visual count of engulfed bacteria, was not significantly increased. The decrease in viability was associated with enhanced intracellular killing of phagocytosed antibiotic-damaged bacteria. Chloramphenicol pretreatment altered the frequency distribution of intracellular bacteria by decreasing the number of leukocytes containing multiple stainable bacteria. Leukocytes failed to kill chloramphenicol-pretreated E. coli in the presence of phenylbutazone, which allowed an accumulation of intracellular bacteria. These results indicate that exposure of E. coli to chloramphenicol renders the bacteria more susceptible to intracellular killing and degradation.
研究了致病性大肠杆菌菌株短期接触氯霉素后对正常人白细胞敏感性的影响。白细胞杀死经氯霉素预处理的大肠杆菌比杀死未处理的对照菌更有效。通过放射性标记细菌的摄取和吞噬细菌的直接视觉计数来衡量，预处理细菌的吞噬作用没有显著增加。活力的降低与吞噬的抗生素损伤细菌的细胞内杀伤增强有关。氯霉素预处理通过减少含有多个可染色细菌的白细胞数量，改变了细胞内细菌的频率分布。在保泰松存在的情况下，白细胞无法杀死经氯霉素预处理的大肠杆菌，这使得细胞内细菌得以积累。这些结果表明，大肠杆菌接触氯霉素会使细菌更容易受到细胞内杀伤和降解。
