Suess U, Pliska V
Brain Res. 1981 Sep 21;221(1):27-33. doi: 10.1016/0006-8993(81)91061-1.
Glia cells of rat neural lobes (pituicytes) were stained in thin sections (6 micrometers) by the indirect immunofluorescence technique for the glial fibrillary acidic protein (GFA), the S-100 protein and fibronectin. The positive strong GFA-staining of the pituicytes demonstrates their astroglial character. Fibronectin was located along the blood capillaries. After six days of implantation of the neural lobes under the kidney capsule of an acceptor rat, the stained fibronectin was augmented and present in a fibrillar network scattered over the tissue; the GFA-staining remained positive, however. The astroglial character of pituicytes was apparently retained in the implantation conditions, whereas neurosecretory axons had already disappeared. No S-100 protein could be unambiguously detected in the glia cells with the antibody employed.
采用间接免疫荧光技术，对大鼠神经叶（垂体细胞）的胶质细胞进行染色，检测其胶质纤维酸性蛋白（GFA）、S-100蛋白和纤连蛋白。垂体细胞GFA染色阳性且强，表明其具有星形胶质细胞特征。纤连蛋白位于毛细血管周围。将神经叶植入受体大鼠肾包膜下六天后，染色的纤连蛋白增加，并呈纤维状网络散布于组织中；然而，GFA染色仍为阳性。在植入条件下，垂体细胞的星形胶质细胞特征显然得以保留，而神经分泌轴突已经消失。使用该抗体未在胶质细胞中明确检测到S-100蛋白。
