将API ZYM系统与API AN-Ident、API 20A、Minitek Anaerobe II和RapID-ANA系统用于艰难梭菌鉴定的比较。
Comparison of API ZYM system with API AN-Ident, API 20A, Minitek Anaerobe II, and RapID-ANA systems for identification of Clostridium difficile.
作者信息
Head C B, Ratnam S
机构信息
Newfoundland Public Health Laboratory, St. John's, Canada.
出版信息
J Clin Microbiol. 1988 Jan;26(1):144-6. doi: 10.1128/jcm.26.1.144-146.1988.
Abstract
The API ZYM system was compared with four anaerobe identification systems for the definitive identification of Clostridium difficile by using 88 cultures of C. difficile grown on Mueller-Hinton blood agar medium. The API ZYM system yielded a distinct and consistent enzyme profile for all test strains, whereas the sensitivities of the other systems in identifying C. difficile ranged from 78 to 96% (AN-Ident, 77.9%; RapID-ANA, 88.6%; Minitek Anaerobe II, 90.9%; and API 20A, 95.5%). The API ZYM system is highly reliable in identifying C. difficile accurately, is rapid, and is relatively simple to use.
摘要
使用在穆勒-欣顿血琼脂培养基上培养的88株艰难梭菌培养物，将API ZYM系统与四种厌氧菌鉴定系统进行比较，以明确鉴定艰难梭菌。API ZYM系统对所有测试菌株产生了独特且一致的酶谱，而其他系统鉴定艰难梭菌的敏感性范围为78%至96%（AN-Ident为77.9%；RapID-ANA为88.6%；Minitek Anaerobe II为90.9%；API 20A为95.5%）。API ZYM系统在准确鉴定艰难梭菌方面高度可靠，速度快且使用相对简单。
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