Levis W R, Whalen J J, Powell J A
Clin Exp Immunol. 1976 Mar;23(3):481-90.
A soluble hapten (dinitrobenzene sulphonic acid (DNBSO3)) and particulate antigens consisting of DNCB, DNFB or DNBSO3 complexed with erythrocytes, all induce a specific blastogenic response and lymphokine production in leucocyte cultures from human subjects topically sensitized to dinitrochlorobenzene (DNCB). While low concentrations (50-100 mug/ml) of DNBSO3 could be left in human leucocyte cultures the entire 4 or 5 days of culture and result in reasonable levels of blastogenesis, it was found that consistently higher degrees of blast transformation resulted when DNCB-sensitive leucocytes were exposed to high concentrations of DNBSO3 (500 mug/ml) for a short period (2 hr). Cell-free supernatants from DNCB-sensitive leucocyte cultures harvested after 48 hr induced blastogenesis and DNA synthesis in secondary target leucocyte cultures from subjects not sensitized to DNCB. Such a blastogenic factor or lymphokine appeared to stimulate even in the absence of any residual antigen, since DNCB complexed to erythrocytes was removed by simple filtration through a 0-45 mum Millipore filter. In contrast to DNCB complexes, the antigenic activity of DNBSO3 complexed with erythrocytes was not removed by such filtration. Thus, several DNP-containing haptens (DNCB, DNFB, DNBSO3) induce specific lymphocyte transformation and lymphokine production when exposed in several different manners to leucocytes from humans sensitized to DNCB. The ability to use either a particulate or soluble stimulant in vitro offers a versatile system for studying cell-mediated immunity in humans with a broad range of potential applicability in both investigate and clinical medicine.
一种可溶性半抗原（二硝基苯磺酸（DNBSO3））以及由二硝基氯苯（DNCB）、二硝基氟苯（DNFB）或与红细胞复合的DNBSO3组成的颗粒性抗原，均可在对二硝基氯苯（DNCB）进行局部致敏的人类受试者的白细胞培养物中诱导特异性的增殖反应和淋巴因子产生。虽然在人类白细胞培养物的整个4或5天培养过程中可留存低浓度（50 - 100μg/ml）的DNBSO3，并产生合理水平的增殖，但发现当DNCB敏感的白细胞短时间（2小时）暴露于高浓度的DNBSO3（500μg/ml）时，增殖转化程度始终更高。48小时后收获的DNCB敏感白细胞培养物的无细胞上清液，可在未对DNCB致敏的受试者的二级靶白细胞培养物中诱导增殖和DNA合成。即使在没有任何残留抗原的情况下，这种增殖因子或淋巴因子似乎也能发挥刺激作用，因为与红细胞复合的DNCB通过0.45μm的密理博滤膜简单过滤即可去除。与DNCB复合物不同，与红细胞复合的DNBSO3的抗原活性不能通过这种过滤去除。因此，几种含二硝基苯基的半抗原（DNCB、DNFB、DNBSO3）在以几种不同方式暴露于对DNCB致敏的人类白细胞时，均可诱导特异性淋巴细胞转化和淋巴因子产生。在体外使用颗粒性或可溶性刺激物的能力，为研究人类细胞介导的免疫提供了一个通用系统，在基础研究和临床医学中具有广泛的潜在应用价值。
