Lantz C H, Lawrie D J, Witebsky F G, MacLowry J D
J Clin Microbiol. 1980 Oct;12(4):583-9. doi: 10.1128/jcm.12.4.583-589.1980.
Four methods for the measurement of serum gentamicin concentration were evaluated with respect to cost-effectiveness, accuracy, and precision. Gentamicin concentration was determined in 112 clinical samples by the Staphlococcus epidermidis agar diffusion bioassay procedure in routine service in our laboratory at the time this study was initiated. Appropriate portions of these clinical samples were frozen and later thawed for remeasurement of gentamicin by bioassay or for measurement of gentamicin in one of three other systems. These included the Enzymatic Radiochemical Assay, the Diagnostic Products Corporation Radioimmunoassay and the New England Nuclear Corporation Radioimmunoassay. In addition, gentamicin dissolved in horse serum at 2, 4, 6, 8, 10, and 12 micrograms/ml was aliquoted, frozen, and later thawed for assay in each of the above systems. The data were analyzed for evidence of constant and proportional bias as well as for accuracy and precision.
就成本效益、准确性和精密度而言，对四种测量血清庆大霉素浓度的方法进行了评估。在本研究开始时，通过表皮葡萄球菌琼脂扩散生物测定法在我们实验室的常规服务中对112份临床样本中的庆大霉素浓度进行了测定。将这些临床样本的适当部分冷冻，之后解冻，以便通过生物测定法重新测量庆大霉素，或在其他三个系统之一中测量庆大霉素。这三个系统包括酶促放射化学测定法、诊断产品公司放射免疫测定法和新英格兰核公司放射免疫测定法。此外，将溶解在马血清中浓度为2、4、6、8、10和12微克/毫升的庆大霉素进行分装、冷冻，之后解冻，以便在上述每个系统中进行测定。对数据进行了分析，以寻找恒定偏差和比例偏差的证据以及准确性和精密度。
