Kiyono H, McGhee J R, Wannemuehler M J, Michalek S M
J Exp Med. 1982 Feb 1;155(2):605-10. doi: 10.1084/jem.155.2.605.
Daily gastric intubation of lipopolysaccharide (LPS)-responsive C3H/HeN, BALB/c, and Swiss mice with SRBC for 2 wk resulted in oral tolerance, whereas similarly treated LPS-nonresponsive C3H/HeJ mice gave splenic anti-SRBC PFC responses, including the IgA isotype, after systemic challenge with antigen. Oral tolerance in LPS-responsive C3H/HeN mice was due to T suppressor (Ts) cells because significant Ts cell activity was demonstrated in both Peyer's patches (PP) and spleens of these animals. On the other hand, T cells from PP and spleens of identically treated C3H/HeJ mice exhibited mainly T helper cell activity. Prior treatment of PP or spleen cell preparations from tolerant C3H/HeN mice with anti-Lyt-2.1 resulted in good in vitro anti-SRBC PFC responses, especially IgA isotype responses in PP cell cultures. These results indicate that oral administration of a thymic-dependent antigen (SRBC) to LPS-responsive mice induced a Ts cell population in PP, which, after migration to peripheral lymphoid tissue (e.g., spleen), suppressed responses to systemically administered antigen. LPS-nonresponsive mice lack this Ts cell pathway and continually respond to oral administration of antigen.
每天给对脂多糖（LPS）有反应的C3H/HeN、BALB/c和瑞士小鼠经胃插管注入绵羊红细胞（SRBC），持续2周，可诱导口服耐受，而同样处理的对LPS无反应的C3H/HeJ小鼠在经抗原全身攻击后，脾脏产生抗SRBC的空斑形成细胞（PFC）反应，包括IgA同种型反应。LPS反应性C3H/HeN小鼠的口服耐受是由于T抑制细胞（Ts），因为在这些动物的派尔集合淋巴结（PP）和脾脏中均显示出显著的Ts细胞活性。另一方面，经同样处理的C3H/HeJ小鼠的PP和脾脏中的T细胞主要表现出T辅助细胞活性。用抗Lyt-2.1预先处理来自耐受C3H/HeN小鼠的PP或脾细胞制剂，可在体外产生良好的抗SRBC PFC反应，尤其是PP细胞培养物中的IgA同种型反应。这些结果表明，给LPS反应性小鼠口服胸腺依赖性抗原（SRBC）可在PP中诱导产生Ts细胞群，该细胞群迁移至外周淋巴组织（如脾脏）后，可抑制对全身给予抗原的反应。LPS无反应性小鼠缺乏这种Ts细胞途径，并且持续对口服抗原产生反应。
