用于鉴定葡萄球菌和微球菌的微量方法组合（API葡萄球菌鉴定系统）的评估
Evaluation of a micromethod gallery (API Staph) for the identification of staphylococci and micrococci.
作者信息
Marples R R, Richardson J F
出版信息
J Clin Pathol. 1982 Jun;35(6):650-6. doi: 10.1136/jcp.35.6.650.
Abstract
A collection of 300 well-characterised strains of staphylococci and micrococci was examined by a commercially available gallery micromethod (API Staph). The results were compared with biotyping by conventional methods. The gallery micromethod broadly agreed with the biotyping scheme used but gave an identification from the index supplied in less than 30% of the trials. Reproducibility was better after 48 h incubation than after 24 h but was poor for the tests for phosphatase and acetoin. When compared with the results of conventional tests, the tests for acetoin, phosphatase and urea were unsatisfactory.
摘要
采用市售的微量鉴定板法（API葡萄球菌鉴定系统）对300株特征明确的葡萄球菌和微球菌进行检测。将结果与传统方法的生物分型结果进行比较。微量鉴定板法与所用的生物分型方案大致相符，但在不到30%的试验中，根据所提供的索引无法鉴定出来。培养48小时后的重现性比24小时后更好，但磷酸酶和3-羟基丁酮试验的重现性较差。与传统试验结果相比，3-羟基丁酮、磷酸酶和尿素试验的结果并不理想。
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