Lee W L, Shalita A R, Suntharalingam K, Fikrig S M
Infect Immun. 1982 Jan;35(1):71-8. doi: 10.1128/iai.35.1.71-78.1982.
The chemoattraction of Propionibacterium acnes lipase for neutrophils and the effect of lipase inhibitor and two antibiotic agents on the chemotaxis were evaluated. Of the various fractions tested, partially purified lipase (fraction 2c) was the most active cytotaxin produced by P. acnes. Serum mediators were not required for the generation of chemotaxis by lipase in vitro. Diisopropyl phosphofluoridate at low concentration (10(-4) mM) completely inhibited lipase activity as well as polymorphonuclear leukocyte chemotaxis generated by lipase. Tetracycline hydrochloride and erythromycin base at concentrations of 10(-1) mM and 1 mM, respectively, caused 100% inhibition of PMN migration toward lipase or zymosan-activated serum. The inhibiting activity of the antibiotics was directed against cells independently of any effect on lipase. Chemotaxis by P. acnes lipase suggests a wider role for this enzyme in the inflammatory process and the pathogenesis of acne vulgaris.
评估了痤疮丙酸杆菌脂肪酶对中性粒细胞的化学吸引作用以及脂肪酶抑制剂和两种抗生素对趋化性的影响。在所测试的各种组分中，部分纯化的脂肪酶（组分2c）是痤疮丙酸杆菌产生的最具活性的细胞趋化素。体外脂肪酶产生趋化性不需要血清介质。低浓度（10^(-4) mM）的二异丙基氟磷酸酯完全抑制脂肪酶活性以及由脂肪酶产生的多形核白细胞趋化性。盐酸四环素和红霉素碱的浓度分别为10^(-1) mM和1 mM时，可100%抑制中性粒细胞向脂肪酶或酵母聚糖激活血清的迁移。抗生素的抑制活性针对细胞，与对脂肪酶的任何作用无关。痤疮丙酸杆菌脂肪酶的趋化性表明该酶在炎症过程和寻常痤疮发病机制中具有更广泛的作用。
