人多形核白细胞对赫氏疏螺旋体吞噬作用的调理素需求。

Opsonic requirements for phagocytosis of Borrelia hermsii by human polymorphonuclear leukocytes.

作者信息

Spagnuolo P J, Butler T, Bloch E H, Santoro C, Tracy J W, Johnson R C

出版信息

J Infect Dis. 1982 Mar;145(3):358-64. doi: 10.1093/infdis/145.3.358.

DOI:10.1093/infdis/145.3.358

Abstract

Opsonic requirements for phagocytosis by human polymorphonuclear leukocytes (PMNLs) of a laboratory strain of Borrelia hermsii were examined. Intracellular localization of spirochetes was confirmed by electron microscopy and cinephotomicroscopy. Phagocytosis of 3H-labeled spirochetes was increased with higher concentrations of pooled human serum or greater ratios of spirochetes to PMNLs and was unaffected by diminished classical complement pathway activity. Immune rabbit serum markedly increased phagocytosis of spirochetes beyond levels observed with preimmune rabbit serum. Immune rabbit serum also demonstrated direct activity against B. hermsii, which decreased the motility and viability and increased agglutination of the spirochetes independently of complement. Both the direct activity against B. hermsii and the opsonic activity of immune rabbit serum were eliminated by immunoabsorption of immunoglobulins. These observations suggest a role for the alternative complement pathway and for immunoglobulins in the opsonization of spirochetes for phagocytosis by PMNLs. Immunoglobulins may also play a role in the direct clearance of spirochetes.

摘要

研究了人多形核白细胞（PMNLs）对实验室菌株赫氏疏螺旋体进行吞噬作用时的调理素需求。通过电子显微镜和电影显微镜确认了螺旋体的细胞内定位。3H标记螺旋体的吞噬作用随着人混合血清浓度的升高或螺旋体与PMNLs比例的增加而增强，并且不受经典补体途径活性降低的影响。免疫兔血清显著增强了螺旋体的吞噬作用，超过了免疫前兔血清所观察到的水平。免疫兔血清还表现出对赫氏疏螺旋体的直接活性，这降低了螺旋体的运动性和活力，并独立于补体增加了螺旋体的凝集。针对赫氏疏螺旋体的直接活性和免疫兔血清的调理活性都通过免疫球蛋白的免疫吸附而消除。这些观察结果表明替代补体途径和免疫球蛋白在螺旋体被PMNLs吞噬的调理过程中发挥作用。免疫球蛋白也可能在螺旋体的直接清除中发挥作用。