Kidd A H, Madeley C R
J Clin Pathol. 1981 Feb;34(2):213-6. doi: 10.1136/jcp.34.2.213.
Sixty-seven stool specimens from 51 children, positive for adenoviruses by electron microscopy and negative for growth in human-embryo kidney cells, were tested for growth in Chang conjunctiva cells. Twenty-eight specimens caused a cytopathic effect over more than one passage in these cultures, and several adenovirus strains grew better at 33 degree C than at 37 degree C. Most of the culture-positive specimens also induced the development of adenovirus antigens in KB cells detectable by a group-specific indirect immunofluorescence test. Twenty-four of the 25 fastidious strains tested were antigenically related and were distinct from the established serotypes commonly isolated from stools.
对51名儿童的67份粪便标本进行检测，这些标本经电子显微镜检查腺病毒呈阳性，在人胚肾细胞中培养呈阴性，检测其在张氏结膜细胞中的生长情况。28份标本在这些培养物中传代一次以上引起细胞病变效应，并且几种腺病毒株在33℃比在37℃生长得更好。大多数培养阳性标本在KB细胞中也诱导了腺病毒抗原的产生，可通过群特异性间接免疫荧光试验检测到。所检测的25株苛求菌中有24株在抗原性上相关，并且与通常从粪便中分离出的已知血清型不同。
