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临床样本的直接气-液色谱法能否检测出特定生物体?

Can direct gas-liquid chromatography of clinical samples detect specific organisms?

作者信息

Watt B, Geddes P A, Greenan O A, Napier S K, Mitchell A

出版信息

J Clin Pathol. 1982 Jul;35(7):706-8. doi: 10.1136/jcp.35.7.706.

Abstract

A total of 1929 samples was analyzed by direct gas-liquid chromatography and the volatile fatty acid (VFA) patterns of the positive samples were compared with the results of culture. There was no correlation between any bacterial genus or species and the detailed VFA patterns although the presence of butyric or valeric acids, or both, was generally associated with the presence of anaerobes and that of acetic acid was generally associated with aerobic bacteria; however, the technique could not predict the nature of the subsequent bacterial isolate. There was also poor correlation between the VFA pattern in a given sample and the VFA pattern(s) of anaerobic bacteria subsequently isolated from that sample.

摘要

通过直接气液色谱法共分析了1929个样本,并将阳性样本的挥发性脂肪酸(VFA)模式与培养结果进行了比较。尽管丁酸或戊酸或两者的存在通常与厌氧菌的存在相关,而乙酸的存在通常与需氧菌相关,但任何细菌属或种与详细的VFA模式之间均无相关性;然而,该技术无法预测后续分离出的细菌的性质。给定样本中的VFA模式与随后从该样本中分离出的厌氧细菌的VFA模式之间的相关性也很差。

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Diagnosis of anaerobic infection by gas chromatographic estimation of volatile fatty acids.
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