Tantravahi R, Miller D A, Dev V G, Miller O J
Chromosoma. 1976 Jun 30;56(1):15-27. doi: 10.1007/BF00293725.
Nucleolus organizer regions were detected by the Ag-AS silver method in fixed metaphase chromosomes from human and primates. In the human, silver was deposited in the secondary constriction of a maximum of five pairs of acrocentric chromosomes: 13, 14, 15, 21 and 22. The chimpanzee also had five pairs of acrocentric chromosomes stained, corresponding to human numbers 13, 14, 18, 21 and 22. A gibbon had a single pair of chromosomes with a secondary constriction, which corresponded to the nucleolus organizer region. In each case the Ag-AS method detected the sites which have been shown by in situ hybridization to contain the ribosomal RNA genes. An orangutan had eight pairs of acrocentric chromosomes stained with Ag-AS, probably corresponding to human numbers 13, 14, 15, 18, 21 and 22, plus two others. Two gorillas had silver stain over two pairs of small acrocentric chromosomes and at the telomere of one chromosome 1. The larger gorilla acrocentric chromosomes had no silver stain although they all had secondary constrictions and entered into satellite associations.
采用银染 - 银增强（Ag - AS）法在人类和灵长类动物固定的中期染色体中检测核仁组织区。在人类中，银沉积在最多五对近端着丝粒染色体的次缢痕处：13、14、15、21和22号染色体。黑猩猩也有五对着丝粒染色体被染色，对应于人类的13、14、18、21和22号染色体。一只长臂猿有一对带有次缢痕的染色体，其对应于核仁组织区。在每种情况下，银染 - 银增强法都检测到了经原位杂交显示含有核糖体RNA基因的位点。一只猩猩有八对着丝粒染色体被银染 - 银增强法染色，可能对应于人类的13、14、15、18、21和22号染色体，另外还有两对。两只大猩猩在两对小的近端着丝粒染色体以及一条1号染色体的端粒处有银染。较大的大猩猩近端着丝粒染色体虽然都有次缢痕并参与随体联合，但没有银染。
