Belshe R B, Van Voris L P, Mufson M A, Buynak E B, McLean A A, Hilleman M A
Infect Immun. 1982 Jul;37(1):160-5. doi: 10.1128/iai.37.1.160-165.1982.
The sensitivity of an enzyme-linked immunosorbent assay (ELISA) to detect low levels of antibody to respiratory syncytial (RS) virus was compared with a tube dilution neutralization test (NEUT) on sera obtained from children who received a parenteral live RS virus vaccine. Among the children who developed antibody in response to live RS virus vaccine. ELISA was as sensitive as NEUT at detecting antibody increases. Some children who did not have detectable prevaccine ELISA antibody possessed NEUT antibody; these children were generally less than 12 months old, suggesting that they had low levels of maternal antibody. Low levels of NEUT or ELISA antibody were associated with the absence of antibody increases after injection of live RS virus vaccine. The quantity of antibody stimulated by this live RS virus vaccine was small compared with that which was stimulated by naturally acquired RS virus infection. We concluded that ELISA is a satisfactory test for determining antibody to RS virus in vaccine field trials, given the understanding that low levels of preexisting antibody are not detected in some instances.
将酶联免疫吸附测定（ELISA）检测呼吸道合胞（RS）病毒低水平抗体的敏感性，与针对接受肠外活RS病毒疫苗儿童血清进行的试管稀释中和试验（NEUT）作了比较。在因活RS病毒疫苗产生抗体的儿童中，ELISA在检测抗体增加方面与NEUT一样敏感。一些接种疫苗前ELISA抗体检测不到的儿童拥有NEUT抗体；这些儿童一般不到12个月大，提示他们的母源抗体水平较低。低水平的NEUT或ELISA抗体与注射活RS病毒疫苗后抗体未增加相关。与自然获得的RS病毒感染所刺激产生的抗体量相比，这种活RS病毒疫苗所刺激产生的抗体量较少。我们得出结论，鉴于在某些情况下检测不到低水平的预先存在的抗体，ELISA对于疫苗现场试验中测定RS病毒抗体而言是一项令人满意的检测方法。
