呼吸道合胞病毒特异性抗体的简易酶联免疫吸附测定法
Simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus.
作者信息
Vaur L, Agut H, Garbarg-Chenon A, Prud'Homme de Saint-Maur G, Nicolas J C, Bricout F
出版信息
J Clin Microbiol. 1986 Oct;24(4):596-9. doi: 10.1128/jcm.24.4.596-599.1986.
A simplified and reliable enzyme-linked immunosorbent assay (ELISA) was applied to the detection of serum antibodies against respiratory syncytial virus (RSV). RSV-infected cells were fixed and dried on 96-well microtiter plates and kept at 4 degrees C. The titers of reference sera were determined by endpoint dilution. A linear relation was found between the titers and the logarithm of absorbance values of sera diluted to 1:1,000 (r = 0.93, P less than 0.001). Measurement of RSV antibodies was done by using a single serum dilution (1:1,000) in conjunction with a standard curve. A strong correlation was found between complement fixation and ELISA results (r = 0.89, P less than 0.001). In addition, the ELISA method exhibited higher titers and a greater sensitivity than did complement fixation, although the applicability of the assay is limited with positive serum samples of low titer.
一种简化且可靠的酶联免疫吸附测定法（ELISA）被用于检测血清中抗呼吸道合胞病毒（RSV）的抗体。将感染RSV的细胞固定并干燥在96孔微量滴定板上，保存在4℃。通过终点稀释法测定参考血清的滴度。发现滴度与稀释至1:1000的血清吸光度值的对数之间存在线性关系（r = 0.93，P < 0.001）。通过使用单一血清稀释度（1:1000）结合标准曲线来测定RSV抗体。发现补体结合试验和ELISA结果之间存在很强的相关性（r = 0.89，P < 0.001）。此外，ELISA方法比补体结合试验显示出更高的滴度和更高的灵敏度，尽管该测定法对于低滴度阳性血清样本的适用性有限。
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