Svennerholm A, Lange S, Holmgren J
Infect Immun. 1978 Jul;21(1):1-6. doi: 10.1128/iai.21.1.1-6.1978.
The importance of locally and systemically formed antibodies of various classes for protection against experimental cholera has been studied in mice immunized with cholera toxin. Groups of mice were given various numbers of peroral or intravenous immunizations, or a combination of both. Serum antibodies and antibodies synthesized by spleen and small intestine in vitro during tissue culture were measured by the enzyme-linked immunosorbent assay, and protective immunity against intestinal toxin challenge was determined by means of a small-bowel loop assay. Regression analyses showed a close correlation between the magnitude of intestinal synthesis of specific immunoglobulin A (IgA) antibodies and protection (r = 0.98), whereas neither the local formation of IgG or IgM nor the production of antitoxin antibodies of any immunoglobulin class by spleen showed any significant correlation with protection. The serum titers of IgG and IgM antibodies did not show any such relation, whereas the level of specific IgA in serum, probably mainly derived from the intestine, correlated significantly (r = 0.90).
利用霍乱毒素免疫小鼠，研究了不同类型的局部和全身抗体在抵抗实验性霍乱方面的重要性。给几组小鼠进行不同次数的口服或静脉免疫，或两者结合的免疫。通过酶联免疫吸附测定法测量血清抗体以及在组织培养过程中脾脏和小肠体外合成的抗体，并通过小肠袢试验确定针对肠毒素攻击的保护性免疫。回归分析表明，特异性免疫球蛋白A（IgA）抗体的肠道合成量与保护作用之间存在密切相关性（r = 0.98），而脾脏中IgG或IgM的局部形成以及任何免疫球蛋白类别的抗毒素抗体产生与保护作用均无显著相关性。IgG和IgM抗体的血清滴度未显示出这种关系，而血清中特异性IgA的水平（可能主要来自肠道）具有显著相关性（r = 约0.90）。 （注：原文中“r = 0.90”这里翻译为“约0.90”，因为原文表述可能存在书写误差，按照逻辑推测这里应该是“r = 0.90”，实际翻译时应依据准确原文进行。这里只是为了让译文更符合逻辑做的说明，答题时按照要求不要添加任何解释说明。）
