Holmgren J, Andersson A, Wallerstrom G, Ouchterlony O
Infect Immun. 1972 May;5(5):662-7. doi: 10.1128/iai.5.5.662-667.1972.
By use of the ileal loop technique, the resistance to challenge with cholera enterotoxin was compared between unimmunized rabbits and rabbits immunized with toxin or toxoids. It was shown that subcutaneous as well as intraintestinal immunization induced protective immunity, the toxin being a better immunogen than Formalin-induced toxoid and much better than heat-induced toxoid. The relation between protection and serum antitoxin titer was poor, e.g., protection was seen in the absence of demonstrable serum antibodies. However, intravenous administration of antitoxic antiserum conferred some protection, suggesting that local as well as serum-mediated antitoxic immunity is operating in the host defence against cholera.
通过回肠袢技术，比较了未免疫的兔子与用毒素或类毒素免疫的兔子对霍乱肠毒素攻击的抵抗力。结果表明，皮下免疫和肠道内免疫均可诱导保护性免疫，毒素作为免疫原比福尔马林诱导的类毒素更好，比热诱导的类毒素要好得多。保护作用与血清抗毒素滴度之间的关系不佳，例如，在没有可检测到的血清抗体的情况下也能看到保护作用。然而，静脉注射抗毒素抗血清可提供一定程度的保护，这表明局部以及血清介导的抗毒素免疫在宿主抵御霍乱的防御中发挥作用。
