影响针对明尼苏达沙门氏菌粗糙突变株免疫球蛋白G抗体的酶联免疫吸附测定的参数。
Parameters affecting the enzyme-linked immunosorbent assay of immunoglobulin G antibody to a rough mutant of Salmonella minnesota.
作者信息
Bruins S C, Ingwer I, Zeckel M L, White A C
出版信息
Infect Immun. 1978 Sep;21(3):721-8. doi: 10.1128/iai.21.3.721-728.1978.
Abstract
An enzyme-linked immunosorbent assay was developed to detect rabbit immunoglobulin G antibodies against purified lipopolysaccharide of the rough Re mutant of Salmonella minnesota. The time necessary for each step was investigated, and this resulted in a test that could be completed in 1 working day. Serial dilutions of rabbit sera drawn throughout immunization with the Re mutant revealed an elimination of the prozone effect upon prolonged immunization. We interpret this to demonstrate an increase in immunoglobulin G affinity for the lipopolysaccharide on prolonged immunization. We propose that the enzyme-linked immunosorbent assay prozone effect be investigated for use as a measure of antibody affinity.
摘要
开发了一种酶联免疫吸附测定法，用于检测兔抗明尼苏达沙门氏菌粗糙Re突变体纯化脂多糖的免疫球蛋白G抗体。研究了每个步骤所需的时间，结果得到了一种可在1个工作日内完成的检测方法。在用Re突变体进行全程免疫期间抽取的兔血清系列稀释液显示，长时间免疫后前带效应消除。我们认为这表明长时间免疫后免疫球蛋白G对脂多糖的亲和力增加。我们建议研究酶联免疫吸附测定法的前带效应，以用作抗体亲和力的一种衡量指标。
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本文引用的文献
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