新型抗生素，烯胺霉素A、B和C。I. 产生菌、发酵及分离

New antibiotics, enaminomycins A, B and C. I. Producing organism, fermentation and isolation.

作者信息

Arai M, Itoh Y, Enokita R, Takamatsu Y, Manome T

出版信息

J Antibiot (Tokyo). 1978 Sep;31(9):829-33. doi: 10.7164/antibiotics.31.829.

DOI:10.7164/antibiotics.31.829

Abstract

New antibiotics, enaminomycins A, B and C, were found in the culture broth of streptomycete strain No. 13120, which was identified as Streptomyces baarnensis and designated as S. baarnensis No. 13120. Fermentation of enaminomycins were performed by conventional submerged culture in a 30-liter jar fermentor. Isolation of the antibiotics was performed by centrifugation of the culture broth and adsorption of the antibiotics from the supernatant on a column of activated carbon, followed by elution with aqueous acetone. Enaminomycins A, B and C were separated from each other on a column of Sephadex LH-20.

摘要

新型抗生素烯胺霉素A、B和C是从链霉菌菌株13120的培养液中发现的，该菌株被鉴定为巴恩链霉菌，并命名为巴恩链霉菌13120。烯胺霉素的发酵是在30升的罐式发酵罐中通过传统的深层培养进行的。抗生素的分离是通过对培养液进行离心，然后将上清液中的抗生素吸附到活性炭柱上，接着用丙酮水溶液洗脱来完成的。烯胺霉素A、B和C在葡聚糖凝胶LH-20柱上彼此分离。