Anger B, Bockman R, Andreeff M, Erlandson R, Jhanwar S, Kameya T, Saigo P, Wright W, Beattie E J, Oettgen H F, Old L J
Cancer. 1982 Oct 15;50(8):1518-29. doi: 10.1002/1097-0142(19821015)50:8<1518::aid-cncr2820500812>3.0.co;2-r.
Two permanent cell lines, designated LU-65 and SK-Luci-6, were established from large-cell anaplastic lung cancers of two patients. Both cell lines grew as solid tumors in nude mice. The histologic pattern of the tumors in the nude mouse resembled that of the primary lung cancers in that the xeno-transplanted tissues showed no distinctive features indicative of cell type, a finding consistent with origin from a large-cell anaplastic lung cancer. Cells from both lines formed clones in semisolid agar. Flow cytometric analysis of SK-Luci-6 showed a hypertriploid stemline with a very high RNA-index. Line LU-65 had a hyperdiploid stemline evolving into a hypertriploid stemline with a high RNA-index. Chromosome analysis showed aneuploidy with abnormalities and marker chromosomes in both tumor cell lines. The isoenzyme pattern of LU-65 and Sk-Luci-6 indicated that they were of human origin and distinct from HeLa cells or another common contaminating line. Both cell lines released biologically active agents that could have caused the neutrophilia and hypercalcemia seen in the patients.
从两名患者的大细胞间变性肺癌中建立了两个永久细胞系，分别命名为LU-65和SK-Luci-6。这两个细胞系在裸鼠体内均长成实体瘤。裸鼠体内肿瘤的组织学模式与原发性肺癌相似，即异种移植组织未显示出指示细胞类型的独特特征，这一发现与源自大细胞间变性肺癌的起源一致。两个细胞系的细胞均在半固体琼脂中形成克隆。对SK-Luci-6的流式细胞术分析显示出具有非常高RNA指数的超三倍体主干系。LU-65系具有超二倍体主干系，逐渐演变成具有高RNA指数的超三倍体主干系。染色体分析显示两个肿瘤细胞系均存在非整倍体，伴有异常和标记染色体。LU-65和Sk-Luci-6的同工酶模式表明它们起源于人类，与HeLa细胞或另一种常见的污染细胞系不同。这两个细胞系均释放出可能导致患者出现嗜中性粒细胞增多和高钙血症的生物活性剂。
