Davies C L, Molyneux S G
J Chromatogr. 1982 Aug 13;231(1):41-51. doi: 10.1016/s0378-4347(00)80507-1.
A procedure is described for the determination of plasma catecholamines using reversed-phase, ion-pair high-performance liquid chromatography coupled with electrochemical detection. Optimisation of chromatographic conditions with respect to detector performance and adherence to procedures and precautions described, render the method applicable to both neurochemical research and routine clinical analysis. The limit of quantitative detection of the method was found to be approximately 30 pg per injection for individual catecholamines. A single chromatographic run, providing adequate resolution of each component, could be completed in approximately 12 min.
本文描述了一种使用反相离子对高效液相色谱结合电化学检测来测定血浆儿茶酚胺的方法。关于检测器性能对色谱条件进行优化，并遵循所述的程序和预防措施，使得该方法适用于神经化学研究和常规临床分析。该方法对单个儿茶酚胺的定量检测限约为每次进样30皮克。一次色谱运行能够在大约12分钟内完成，各组分之间有足够的分离度。
