Felgenhauer K, Nekic M, Ackermann R
J Neuroimmunol. 1982 Oct;3(2):149-58. doi: 10.1016/0165-5728(82)90048-0.
A newly developed peroxidase-linked immunoassay is described which is sensitive enough to quantify herpes simplex antibodies in serum and cerebrospinal fluid diluted to an IgG level of 1 mg/dl. Thus, a comparison of photometric signals allows the direct detection of specific antibodies which have been secreted by activated tissue B lymphocytes into the CSF compartment during the humoral immune phase of herpes simplex encephalitis. The technique utilizes urea-Triton-dissolved virus antigens covalently bound to Sepharose 4B pearls. A highly specific sandwich antibody was purified by immune absorption column chromatography and labelled in its protected state. In the majority of cases the antibody level increased around the 10th day, to reach its maximum a few days after. In some cases however the serum levels gradually rose over a period of several weeks. The antibody levels in the CSF increase uniformly at the same time, irrespective of the general immune response and soared up to higher than serum levels within a few days. Local antibody production may persist for years so that late diagnosis of herpes encephalitis becomes possible with a single side by side test of serum and CSF from the patient.
本文描述了一种新开发的过氧化物酶联免疫测定法，该方法灵敏度足以定量检测稀释至IgG水平为1mg/dl的血清和脑脊液中的单纯疱疹抗体。因此，通过比较光度信号，可以直接检测在单纯疱疹性脑炎体液免疫阶段由活化的组织B淋巴细胞分泌到脑脊液中的特异性抗体。该技术利用与琼脂糖4B珠共价结合的尿素- Triton溶解的病毒抗原。通过免疫吸附柱色谱法纯化一种高度特异性的夹心抗体，并在其受保护状态下进行标记。在大多数情况下，抗体水平在第10天左右升高，几天后达到最大值。然而，在某些情况下，血清水平在几周内逐渐上升。脑脊液中的抗体水平同时均匀升高，与总体免疫反应无关，并在几天内飙升至高于血清水平。局部抗体产生可能持续数年，因此通过对患者血清和脑脊液进行单次并行检测，有可能对疱疹性脑炎进行晚期诊断。
