Carter M J, Mahy B W
Arch Virol. 1982;73(2):109-19. doi: 10.1007/BF01314720.
Incomplete influenza A virus (fowl plague Dobson strain) was prepared by undiluted passage in primary chick embryo fibroblast cells. Analysis of released virus RNA revealed a deficiency in RNA segments 1-3, characteristic of incomplete virus formation. The virus yield from a high multiplicity infection with standard virus always showed this deficiency, even when analysed as early as 6 hours post-infection, whereas infection at low multiplicity gave rise to virus indistinguishable in RNA composition from the parent virus. The relative amounts of intracellular, non-polyadenylated, complementary RNA (template RNA) were found to reflect accurately the eventual RNA composition of released virus, and were altered in phase with PFU:HAU ratio, throughout a von Magnus cycle.
通过在原代鸡胚成纤维细胞中不稀释传代制备了不完全甲型流感病毒（禽瘟热多布森毒株）。对释放的病毒RNA进行分析发现，RNA片段1 - 3存在缺陷，这是不完全病毒形成的特征。即使在感染后6小时最早进行分析，用标准病毒进行高感染复数感染产生的病毒产量始终显示出这种缺陷，而低感染复数感染产生的病毒在RNA组成上与亲本病毒无法区分。发现细胞内非多聚腺苷酸化互补RNA（模板RNA）的相对量准确反映了释放病毒最终的RNA组成，并且在整个冯·马格努斯循环中，其与PFU:HAU比值同步变化。
