Moss B A, Brownlee G G
Nucleic Acids Res. 1981 Apr 24;9(8):1941-7. doi: 10.1093/nar/9.8.1941.
A small RNA segment from the influenza virus strain A/NT/60/68 (H3N2) was converted to cDNA and then to double-stranded DNA using synthetic oligodeoxynucleotide primers. The double-stranded form was cloned into the bacteriophage M1 3mp7. Clones yielding single-strand recombinant templates in opposite orientation were sequenced by the Sanger dideoxynucleotide chain termination technique. The small viral RNA was 422 nucleotides long and the evidence indicated that it was formed by internal deletion of segment 3. It also contained sequences homologous to segment 1.
来自甲型流感病毒株A/NT/60/68(H3N2)的一小段RNA被转化为cDNA,然后使用合成寡脱氧核苷酸引物转化为双链DNA。双链形式被克隆到噬菌体M1 3mp7中。通过桑格双脱氧核苷酸链终止技术对产生相反方向单链重组模板的克隆进行测序。这种小病毒RNA长422个核苷酸,证据表明它是由第3节段的内部缺失形成的。它还包含与第1节段同源的序列。