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流感病毒冯·马格努斯颗粒的额外RNA会导致特定聚合酶基因减少。

Extra RNAs of von Magnus particles of influenza virus cause reduction of particular polymerase genes.

作者信息

Ueda M, Nakajima K, Sugiura A

出版信息

J Virol. 1980 Apr;34(1):1-8. doi: 10.1128/JVI.34.1.1-8.1980.

DOI:10.1128/JVI.34.1.1-8.1980
PMID:7373706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288665/
Abstract

Extra RNAs, or RNA species other than eight gene segments, in von Magnus particles of the influenza virus WSN strain were studied by polyacrylamide gel electrophoresis and oligonucleotide mapping. From the original virus stock, various cloned stocks were obtained, each giving rise to a characteristic set of extra RNAs. One cloned virus stock contained a large number of von Magnus particles. The RNA pattern was characterized by two prominent extra RNAs (X1 and X2) and a decrease in the content of two polymerase genes, P1 and P2. Segregation of the two extra RNAs was carried out by coinfection of cells with a von Magnus particle and infectious virions. The results showed that the presence of one of the extra RNAs (X2) was associated with a reduction in the amount of the P1 gene and that the presence of the other extra RNA (X1) was associated with a reduction in the amount of the P2 gene. Oligonucleotide mapping showed that both extra RNAs, X1 and X2, were derived from the P1 gene. The results suggested that an extra RNA did not necessarily cause the reduction of the progenitor polymerase gene, but might cause the reduction of another polymerase gene.

摘要

通过聚丙烯酰胺凝胶电泳和寡核苷酸图谱分析，对流感病毒WSN株的冯·马格努斯颗粒中的额外RNA（即除八个基因片段以外的RNA种类）进行了研究。从原始病毒毒株中获得了各种克隆毒株，每种克隆毒株都产生一组独特的额外RNA。其中一种克隆病毒毒株含有大量冯·马格努斯颗粒。其RNA图谱的特征是有两条显著的额外RNA（X1和X2）以及两种聚合酶基因P1和P2的含量减少。通过用冯·马格努斯颗粒和感染性病毒粒子共同感染细胞，对这两条额外RNA进行了分离。结果表明，其中一条额外RNA（X2）的存在与P1基因数量的减少有关，而另一条额外RNA（X1）的存在与P2基因数量的减少有关。寡核苷酸图谱分析表明，额外RNA X1和X2均来源于P1基因。结果提示，额外RNA不一定会导致其祖先聚合酶基因数量减少，但可能会导致另一种聚合酶基因数量减少。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/3c77d1f31618/jvirol00172-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/09b34b31a08c/jvirol00172-0010-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/25e8fe561e3c/jvirol00172-0011-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/da76b00de587/jvirol00172-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/3c77d1f31618/jvirol00172-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/09b34b31a08c/jvirol00172-0010-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/25e8fe561e3c/jvirol00172-0011-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/da76b00de587/jvirol00172-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/993e/288665/3c77d1f31618/jvirol00172-0013-a.jpg

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本文引用的文献

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Complete sequence analyses show that two defective interfering influenza viral RNAs contain a single internal deletion of a polymerase gene.完整的序列分析表明，两种缺陷干扰性流感病毒RNA包含一个聚合酶基因的单一内部缺失。
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Temperature-sensitive influenza A virus clones originated by a cross between A/Aichi/2/68 (H3N2) and B/Yamagata/1/73.通过A/爱知/2/68（H3N2）与B/山形/1/73杂交产生的温度敏感甲型流感病毒克隆株。
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Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes.流感病毒基因组图谱：血凝素和神经氨酸酶基因的鉴定。
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