Bersani Amado C A, Garcia Leme J
Br J Exp Pathol. 1982 Oct;63(5):463-71.
Leucopenic rats were injected i.v. with either allogeneic or syngeneic lymphocyte suspensions and their capacity to respond to carrageenin tested at various intervals after the injection of the cells. The carrageenin-induced inflammatory response, which is characteristically attenuated in leucopenic animals, was partially but none the less definitely restored when allogeneic cells were transfused 20 min before the irritant, and less so when they were given 1 or 2 h before. Syngeneic cells were effective in restoring this response up to 2 h after injection. Only a small fraction of the exogenous cells, either allogeneic or syngeneic, remained in the blood, and this for short intervals. One hour after administration of cell suspensions blood leucocyte counts again attained previous values. The same rate of disappearance of the cells from blood was observed in splenectomized rats. Removal of the cells from circulation was thought to reflect "homing" into lymphoid tissues and other tissues, but not selectively in the spleen. Lymphocyte constituents, obtained by cell disintegration and injected i.v. into leucopenic rats, did not cause any change in blood leucocyte counts and yet restored the previously inhibited inflammatory response to carrageenin, as much as the viable cells. It is suggested that inflammation-producing materials, either deposited in tissues or formed or transformed at a site of injury, might gain access to the lymphatics during the early stages of a non-immune, inflammatory response, and "stimulate" lymphoid cells in adjacent lymphoid tissues either to release pro-inflammatory factors or to migrate to the site of injury, where they would exert pro-inflammatory activities. Apparently, only a small number of these cells in involved in the response.
给白细胞减少的大鼠静脉注射同种异体或同基因淋巴细胞悬液，并在注射细胞后的不同时间间隔测试它们对角叉菜胶的反应能力。角叉菜胶诱导的炎症反应在白细胞减少的动物中通常会减弱，当在注入刺激物前20分钟输注同种异体细胞时，该反应会部分但明确地恢复，而在注入前1或2小时给予时恢复程度较小。同基因细胞在注射后2小时内对恢复该反应有效。无论是同种异体还是同基因的外源性细胞，只有一小部分会在血液中短暂停留。给予细胞悬液1小时后，血液白细胞计数再次达到先前水平。在脾切除的大鼠中观察到细胞从血液中消失的速率相同。细胞从循环中清除被认为反映了它们“归巢”到淋巴组织和其他组织中，但不是选择性地进入脾脏。通过细胞裂解获得的淋巴细胞成分静脉注射到白细胞减少的大鼠中，不会引起血液白细胞计数的任何变化，但仍能像活细胞一样恢复先前被抑制的对角叉菜胶的炎症反应。有人提出，在非免疫性炎症反应的早期阶段，沉积在组织中或在损伤部位形成或转化的炎症产生物质可能进入淋巴管，并“刺激”相邻淋巴组织中的淋巴细胞释放促炎因子或迁移到损伤部位，在那里它们将发挥促炎活性。显然，只有少数这些细胞参与了该反应。
