小鼠单克隆抗-N. II。理化特性及常规血型鉴定评估。

Mouse monoclonal anti-N. II. Physicochemical characterization and assessment for routine blood grouping.

作者信息

Fraser R H, Munro A C, Williamson A R, Barrie E K, Hamilton E A, Mitchell R

出版信息

J Immunogenet. 1982 Oct;9(5):303-9. doi: 10.1111/j.1744-313x.1982.tb00986.x.

DOI:10.1111/j.1744-313x.1982.tb00986.x

Abstract

The precise physicochemical conditions under which two examples of mouse monoclonal anti-N can be used as blood grouping reagents have been defined. The antibodies were shown to belong to the IgG1 and IgG2b subclasses respectively and both reacted within discrete ranges of temperature and pH with optimal reactions occurring at 20 degrees C or less and pH 8.5. Under these conditions and at concentrations of 2-3 micrograms/ml, the antibodies were used, in parallel with conventional polyclonal antisera, to type a series of random blood donors. The results obtained with one of the monoclonal antibodies and the polyclonal reagents were in perfect agreement and the monoclonal antibody was judged to have considerable potential as an N-grouping reagent.

摘要

已确定了两种小鼠抗 N 单克隆抗体可用作血型分型试剂的精确物理化学条件。这些抗体分别属于 IgG1 和 IgG2b 亚类，且在不同的温度和 pH 范围内均有反应，最佳反应温度为 20℃或更低，pH 为 8.5。在这些条件下，当抗体浓度为 2 - 3 微克/毫升时，与传统多克隆抗血清平行使用，对一系列随机献血者进行血型分型。其中一种单克隆抗体与多克隆试剂得到的结果完全一致，该单克隆抗体被认为具有作为 N 血型分型试剂的巨大潜力。