Pinheiro F P, Hoch A L, Gomes M L, Roberts D R
Am J Trop Med Hyg. 1981 Jan;30(1):172-6.
Biological transmission of Oropouche (ORO) virus by Culicoides paraensis (Goeldi) has been successfully demonstrated in the laboratory. Adult Culicoides, collected in an area where ORO virus was absent, were infected by feeding on viremic hamsters and then periodically exposed to susceptible hamsters at specific intervals post-infectious blood meal. These C. paraensis were capable of biological transmission of the virus 4-9 days post-feeding on viremic hamsters circulating 6.7-9.9 log10SMLD50/ml virus. Infection rates of 54% and 80% were found for C. paraensis assayed 7 days post-feeding. Virus transmission rates for these experiments were 83% and 25%, respectively. No evidence of mechanical transmission of ORO virus by C. paraensis was observed when interrupted feeding of Culicoides on viremic hamsters was followed by feeding on susceptible hamsters.
在实验室中已成功证明，副库蠓（Goeldi）可进行奥罗普切（ORO）病毒的生物传播。在一个不存在ORO病毒的地区采集成年副库蠓，通过让它们吸食病毒血症仓鼠的血液使其感染，然后在感染性血餐后的特定间隔时间定期将其暴露于易感仓鼠。这些副库蠓在吸食病毒血症仓鼠血液4 - 9天后能够进行病毒的生物传播，此时仓鼠血液中病毒含量为6.7 - 9.9 log10SMLD50/ml。在喂食后7天检测副库蠓时，发现感染率分别为54%和80%。这些实验的病毒传播率分别为83%和25%。当副库蠓在病毒血症仓鼠上中断取食后再喂食易感仓鼠时，未观察到副库蠓对ORO病毒进行机械传播的证据。
