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噬菌体分布研究:哺乳动物粪便中的烈性噬菌体和温和噬菌体含量

Studies on bacteriophage distribution: virulent and temperate bacteriophage content of mammalian feces.

作者信息

Dhillon T S, Dhillon E K, Chau H C, Li W K, Tsang A H

出版信息

Appl Environ Microbiol. 1976 Jul;32(1):68-74. doi: 10.1128/aem.32.1.68-74.1976.

DOI:10.1128/aem.32.1.68-74.1976
PMID:987749
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC170007/
Abstract

Freshly voided samples of the feces of cows, pigs, and humans were analyzed for the enumeration of cell-free plaque-forming units (PFU) of coliphages and Salmonella phages. Coliphage PFU counts per gram (wet weight) of feces were found to range from less than 10(1) to greater than 10(7). Salmonella phages were found in three out of five porcine samples, but none were found in the four bovine samples analyzed. Virulent coliphages related to the phiX174/S13 serological group showed some "habitat preference" in that the S13 type of phages was found only in pig feces, whereas the phiX174 type of phages was found only in cow dung. Temperate coliphages were detectable in a majority of samples of both human and porcine origin but were infrequently found in bovine samples. About 80% of the temperate coliphages of fecal origin have been found to be serologically related to phage HK022 (Dhillon and Dhillon, 1973), and all are efficiently inducible by ultraviolet light irradiation. However, considerable diversity with the group was found when the prophage immunity pattern of 10 randomly selected isolates was examined.

摘要

对刚排出的牛、猪和人的粪便样本进行分析,以计数大肠杆菌噬菌体和沙门氏菌噬菌体的无细胞噬菌斑形成单位(PFU)。发现每克(湿重)粪便中的大肠杆菌噬菌体PFU计数范围从小于10(1)到大于10(7)。在五个猪样本中有三个检测到沙门氏菌噬菌体,但在所分析的四个牛样本中均未发现。与phiX174/S13血清学组相关的烈性大肠杆菌噬菌体表现出一定的“栖息地偏好”,即S13型噬菌体仅在猪粪便中发现,而phiX174型噬菌体仅在牛粪中发现。在大多数人类和猪源样本中可检测到温和性大肠杆菌噬菌体,但在牛样本中很少发现。已发现约80%的粪便来源温和性大肠杆菌噬菌体在血清学上与噬菌体HK022相关(Dhillon和Dhillon,1973),并且所有这些噬菌体都能通过紫外线照射有效诱导。然而,当检查10个随机选择的分离株的原噬菌体免疫模式时,发现该组存在相当大的多样性。

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