全血在中性粒细胞迁移测量中的应用。
Use of whole blood in the measurement of neutrophil migration.
作者信息
Al-Hadithy H, Addison I E, Goldstone A H
出版信息
J Clin Pathol. 1981 Feb;34(2):158-62. doi: 10.1136/jcp.34.2.158.
A whole-blood method was used for measuring neutrophil migration in micropore membrane. The method is reproducible (coefficient of variation (CV) 10.3% and 9.8% in two normal individuals tested repeatedly) and can be performed on an 0.25 ml lithium heparin blood sample. Migration is independent of leucocyte and erythrocyte counts and is comparable with that obtained for separated cells. As in separated cell techniques, cytochalasin and casein respectively inhibit and stimulate neutrophil migration. The technique is of value when testing large numbers of samples and when only small volumes of blood can be obtained, for example, in neonates.
采用全血法测定微孔膜中中性粒细胞的迁移。该方法具有可重复性（在两名正常个体中重复测试，变异系数（CV）分别为10.3%和9.8%），并且可以用0.25 ml肝素锂抗凝血样本进行检测。迁移与白细胞和红细胞计数无关，并且与分离细胞所获得的结果相当。与分离细胞技术一样，细胞松弛素和酪蛋白分别抑制和刺激中性粒细胞迁移。当检测大量样本以及只能获取少量血液时，例如在新生儿中，该技术具有重要价值。
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