Frost J, Cummins H Z
Science. 1981 Jun 26;212(4502):1520-2. doi: 10.1126/science.7233239.
Microscopic methods of performing motility assays of spermatozoa are slow, subjective, and involve a small number of spermatozoa. Laser light-scattering methods can analyze the motility of many spermatozoa within minutes. The swimming speed distribution of human spermatozoa was investigated by photon correlation spectroscopy. The sperm was diluted in seminal plasma to avoid modifying the viscosity. The swimming speed distribution was reconstructed from the correlation data by Stock's method of splines. When compared with a videomicroscopic assay, the reconstructed swimming speed distribution accurately reflects translational motion between 0 and 80 micrometers per second, while for speeds greater than 80 micrometers per second the distribution is distorted by the effects of rotational motion.
对精子进行活力测定的显微镜方法操作缓慢、主观性强，且涉及的精子数量较少。激光散射法可在数分钟内分析许多精子的活力。通过光子相关光谱法研究了人类精子的游动速度分布。将精子在精浆中稀释以避免改变粘度。利用斯托克斯样条法从相关数据重建游动速度分布。与视频显微镜测定法相比，重建的游动速度分布能准确反映每秒0至80微米之间的平移运动，而对于速度大于每秒80微米的情况，该分布会因旋转运动的影响而失真。
