通过光子相关光谱法测量细菌翻译
The measurement of bacterial translation by photon correlation spectroscopy.
作者信息
Stock G B
机构信息
Thomas C Jenkins department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218 USA.
出版信息
Biophys J. 1978 Apr;22(1):79-96. doi: 10.1016/S0006-3495(78)85472-1.
Photon correlation spectroscopy is shown to be a practical technique for the accurate determination of translational speeds of bacteria. Though other attempts have been made to use light scattering as a probe of various aspects of bacterial motility, no other comprehensive studies to establish firmly the basic capabilities and limitations of the technique have been published. The intrinsic accuracy of the assay of translational speeds by photon correlation spectroscopy is investigated by analysis of synthetic autocorrelation data; consistently accurate estimates of the mean and second moment of the speed distribution can be calculated. Extensive analyses of experimental preparations of Salmonella typhimurium examine the possible sources of experimental difficulty with the assay. Cinematography confirms the bacterial speed estimates obtained by photon correlation techniques.
光子相关光谱法被证明是一种用于准确测定细菌平移速度的实用技术。尽管此前已有其他尝试将光散射用作探测细菌运动各方面的手段，但尚未发表其他全面研究以牢固确立该技术的基本能力和局限性。通过对合成自相关数据的分析，研究了光子相关光谱法测定平移速度的内在准确性；可以计算出速度分布的均值和二阶矩的一致准确估计值。对鼠伤寒沙门氏菌实验制剂的广泛分析考察了该测定可能存在的实验困难来源。电影摄影术证实了通过光子相关技术获得的细菌速度估计值。
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