仓鼠线粒体13S核糖体RNA的甲基化状态：一种新型核糖核苷N4-甲基胞苷的存在。
Methylation status of 13S ribosomal RNA from hamster mitochondria: the presence of a novel riboside, N4-methylcytidine.
作者信息
Dubin D T, Taylor R H, Davenport L W
出版信息
Nucleic Acids Res. 1978 Nov;5(11):4385-97. doi: 10.1093/nar/5.11.4385.
Abstract
The ribosomal RNA ("13S" RNA) of the small ribosomal subunit of hamster cell mitochondria has been found to have a distinctive pattern of methylated residues. Each molecule contained, on the average, approximately one residue of m4Cp, m5Cp and m5Up, and two residues of m62Ap. The natural occurrence of m4Cp has not previously been reported; we propose that this nucleotide is homologous to its ribose-methylated congener, m4Cmp, which is characteristic of bacterial 16S ribosomal RNA. We detected neither m4Cp nor m4Cmp in the hamster cell cytoplasmic ribosomal RNA. This is the first documentation of a modified residue present in mitochondrial RNA but absent from the cytoplasmic RNA of the same cells.
摘要
已发现仓鼠细胞线粒体小核糖体亚基的核糖体RNA（“13S”RNA）具有独特的甲基化残基模式。每个分子平均含有约一个m4Cp、m5Cp和m5Up残基，以及两个m62Ap残基。m4Cp的天然存在此前尚未见报道；我们认为该核苷酸与其核糖甲基化同类物m4Cmp同源，m4Cmp是细菌16S核糖体RNA的特征。我们在仓鼠细胞胞质核糖体RNA中未检测到m4Cp和m4Cmp。这是首次记录到线粒体RNA中存在而同一细胞的胞质RNA中不存在的修饰残基。
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