Annen J M, Köhler P, Eckert J
Z Parasitenkd. 1981;65(1):79-88. doi: 10.1007/BF00926556.
In a cytotoxicity assay, using rat spleen cells as target cells, Echinococcus granulosus cyst fluid from cattle exerted a marked degree of cytotoxicity in vitro. When trypan blue exclusion or [3H]thymidine incorporation by concanavalin A stimulated spleen cells was used as a measure of cell viability, dialyzed cyst fluid showed maximum cell destruction up to a 1:8 dilution. The effect was dose and time dependent, cells being affected by 24 h after exposure to cyst fluid. The components responsible for cytotoxicity of cyst fluid were heat stable and could be recovered using gel chromatography on Sephadex G 50 and G 15 as a single low molecular weight fraction. It is assumed that the toxic products released by the living parasite can readily penetrate through the cyst wall into the surrounding host tissue. The interference of such substances with immunocompetent cells might account for the long-term survival of the parasite in the intermediate host.
在一项细胞毒性试验中，以大鼠脾细胞作为靶细胞，牛细粒棘球绦虫囊液在体外表现出显著程度的细胞毒性。当使用锥虫蓝排斥试验或用伴刀豆球蛋白A刺激的脾细胞掺入[3H]胸腺嘧啶核苷作为细胞活力的衡量指标时，透析后的囊液在高达1:8的稀释度下显示出最大程度的细胞破坏。这种效应具有剂量和时间依赖性，细胞在接触囊液后24小时受到影响。导致囊液细胞毒性的成分具有热稳定性，并且可以通过在Sephadex G 50和G 15上进行凝胶色谱法作为单一低分子量组分回收。据推测，活寄生虫释放的有毒产物能够轻易穿透囊壁进入周围的宿主组织。此类物质对免疫活性细胞的干扰可能是寄生虫在中间宿主体内长期存活的原因。
