万古霉素的灵敏放射免疫测定法。
Sensitive radioimmunoassay for vancomycin.
作者信息
Fong K L, Ho D H, Bogerd L, Pan T, Brown N S, Gentry L, Bodey G P
出版信息
Antimicrob Agents Chemother. 1981 Jan;19(1):139-43. doi: 10.1128/AAC.19.1.139.
A radioimmunoassay for vancomycin has been developed which uses rabbit antiserum induced by vancomycin-bovine serum albumin conjugates and vancomycin labeled with 3H or 125I. Using either isotope, the method is simple and reproducible and has a sensitivity of 4 or 0.04 ng/ml, depending on the tracer used. This is 200- to 20,000-fold improvement in sensitivity compared with the most sensitive bioassay. Drug levels in serum or urine samples from patients receiving vancomycin can be determined by this assay procedure without processing. The data obtained with 3H and 125I labels were in good agreement. Patients' plasma vancomycin concentrations determined by radioimmunoassay correlated well with those determined by bioassay when the drug was administered intravenously. However, after oral administration the drug could be detected only by radioimmunoassay. The antiserum was evaluated for cross-reactivity with a wide variety of antibiotics and cancer chemotherapeutic agents, and no significant interference was found.
已开发出一种万古霉素放射免疫测定法，该方法使用由万古霉素-牛血清白蛋白偶联物诱导产生的兔抗血清以及用³H或¹²⁵I标记的万古霉素。使用任何一种同位素，该方法都简单且可重复，根据所使用的示踪剂，灵敏度为4或0.04纳克/毫升。与最灵敏的生物测定法相比，灵敏度提高了200至20000倍。接受万古霉素治疗的患者血清或尿液样本中的药物水平无需处理即可通过该测定程序确定。用³H和¹²⁵I标记获得的数据吻合良好。当静脉给药时，通过放射免疫测定法测定的患者血浆万古霉素浓度与通过生物测定法测定的浓度相关性良好。然而，口服给药后，只能通过放射免疫测定法检测到该药物。评估了抗血清与多种抗生素和癌症化疗药物的交叉反应性，未发现明显干扰。
Zotero 插件