Boudet R A, Morse P A, Watts L M
Surg Gynecol Obstet. 1978 Apr;146(4):609-16.
A population of 80 defined normal people ranging in age from 20 to 62 years were used to define a dose response curve in whole blood phytohemagglutinin blastogenesis of lymphocytes. Maximum stimulation occurred at four days of incubation and 18 hours of pulse labeling with tritiated thymidine using 50 micrograms per milliliter of phytohemagglutinin and 1 microcurie of tritiated thymidine. An analysis of variants indicates a statistically significant trend of reduction of disintegrations per minute per milliliter with advancing age. Likewise, there was an extremely significant difference between measurements performed in the morning and late in the afternoon. Measurements referred to sex and sequential testing were not shown to be significant. Therefore, whole blood phytohemagglutinin blastogenesis may be meaningfully performed and interpreted using nonparametric statistics and the raw blastogenesis of a standard amount of peripheral whole blood, provided that the remainder of the test conditions are rigidly controlled.
选取了80名年龄在20至62岁之间的健康人群，用于确定淋巴细胞全血植物血凝素增殖反应的剂量反应曲线。使用每毫升50微克植物血凝素和1微居里氚标记胸腺嘧啶核苷，在培养四天并进行18小时脉冲标记后出现最大刺激。方差分析表明，随着年龄增长，每毫升每分钟的衰变次数呈统计学上显著的下降趋势。同样，上午和下午晚些时候进行的测量之间存在极其显著的差异。按性别和顺序测试进行的测量未显示出显著差异。因此，只要严格控制测试条件的其余部分，使用非参数统计和标准量外周全血的原始增殖反应，就可以有意义地进行和解释全血植物血凝素增殖反应。
