Mehta P D, Patrick B A, Thormar H
J Clin Microbiol. 1982 Nov;16(5):985-7. doi: 10.1128/jcm.16.5.985-987.1982.
We developed a sensitive peroxidase staining procedure to identify oligoclonal band specificity in subacute sclerosing panencephalitis by subjecting serum and cerebrospinal fluid to isoelectric focusing and immunofixation with measles virus. The gel was washed and stained with horseradish peroxidase-conjugated goat antihuman immunoglobulin G. The samples showed dark brown measles-specific oligoclonal bands in the alkaline pH region of the gel, whereas the controls showed no significant staining. This technique may be useful in identifying specific antibody activity against viral and other antigens in oligoclonal bands in cerebrospinal fluid and serum.
我们开发了一种灵敏的过氧化物酶染色程序，通过对血清和脑脊液进行等电聚焦以及用麻疹病毒进行免疫固定，来鉴定亚急性硬化性全脑炎中的寡克隆带特异性。将凝胶洗涤后，用辣根过氧化物酶偶联的山羊抗人免疫球蛋白G进行染色。样品在凝胶的碱性pH区域显示出深棕色的麻疹特异性寡克隆带，而对照则无明显染色。该技术可能有助于鉴定脑脊液和血清中寡克隆带针对病毒及其他抗原的特异性抗体活性。
