Tung K S, DeHoratius R J, Williams R C
Clin Exp Immunol. 1981 Mar;43(3):615-25.
The molecular size of circulating immune complexes in patients with systemic lupus erythematosus was determined by the C1q solid-phase assay after the sera were fractionated by sucrose-gradient ultracentrifugation. Circulating immune complexes in patients with membranous glomerulonephritis were uniformly large, sedimenting exclusively above 19S, whereas the immune complexes in patients with cerebritis were small, at or just above 7S. In lupus patients with diffuse proliferative glomerulonephritis and patients without renal involvement, immune complexes of both large and small sizes were found. Of patients without renal involvement, more circulating immune complexes were associated with active disease (n = 22, prevalence = 82%, mean level = 24 standard deviations) than with inactive disease (n = 17, prevalence = 41%, mean level = 41%, mean level = 6 . 5 standard deviations). In patients with clinical evidence for renal involvement, circulating immune complexes were detected in all of five patients with membranous glomerulonephritis, in 88% of 17 patients with diffuse proliferative glomerulonephritis and in one of four patients with mesangial nephritis. Thus, in addition to the finding of an overall positive correlation between disease activity and circulating immune complex levels, circulating immune complexes of certain general molecular size ranges appear to be associated with different clinical manifestations of systemic lupus erythematosus.
在通过蔗糖梯度超速离心对血清进行分级分离后，采用C1q固相分析法测定系统性红斑狼疮患者循环免疫复合物的分子大小。膜性肾小球肾炎患者的循环免疫复合物均较大，仅在19S以上沉降，而脑炎患者的免疫复合物较小，在7S或略高于7S。在患有弥漫性增殖性肾小球肾炎的狼疮患者和无肾脏受累的患者中，均发现了大小不同的免疫复合物。在无肾脏受累的患者中，与活动性疾病相关的循环免疫复合物更多（n = 22，患病率 = 82%，平均水平 = 24个标准差），而非活动性疾病患者（n = 17，患病率 = 41%，平均水平 = 6.5个标准差）。在有肾脏受累临床证据的患者中，5例膜性肾小球肾炎患者全部检测到循环免疫复合物，17例弥漫性增殖性肾小球肾炎患者中有88%检测到，4例系膜性肾炎患者中有1例检测到。因此，除了发现疾病活动与循环免疫复合物水平之间存在总体正相关外，某些一般分子大小范围的循环免疫复合物似乎与系统性红斑狼疮的不同临床表现有关。
