Teratocarcinoma transplantation rejection loci: genetic localization of the Gt-1 locus on chromosome 17 and the expression of alternate alleles.

A series of congenic mice on the BALB/c genetic background have been employed to localize a teratocarcinoma transplantation rejection locus, Gt-1, to the K side of the H-2 locus on chromosome 17. Previous studies have placed the Gt-1sv allele about 8 centimorgans away from the H-2b or H-2bv1 locus. Teratocarcinomas derived from 129/sv mice, Gt-1sv (H-2Kbv1/H-2Dbv1), are rejected by BALB/c(H-2Kd/H-2Dd) and BALB.G mice (H-2Kd/H2D-b, but form tumors in BALB.B (H-2Kb/H2Db) and BALB/5R5 mice (H-2Kb/H2Dd). In the reciprocal tumor-rejection test, a BALB/c teratocarcinoma was rejected by immunized BALB.B mice, but formed tumors in the immunized isogenic BALB/c mouse. These studies demonstrate the reciprocal expression of two Gt-1 alleles, one Gt-1c, in BALB/c mice, and the other, Gt-1sv, in the congenic BALB.B mice, Shedlovsky and co-workers have placed the GT-1 locus in a similar location on the K side of the H-2 locus on chromosome 17.