Muirhead E E, Byers L W, Desiderio D M, Pitcock J A, Brooks B, Brown P S, Brosius W L
J Lab Clin Med. 1982 Jan;99(1):64-75.
The ANRL was derived from the renal venous effluent as the kidney exerted its nonexcretory antihypertensive function. This was made possible by three developments: (1) improvement in the extraction of ANRL from fresh renal medulla; (2) the fact that purified ANRL caused an acute vasodepressor effect (acted as a vasodilator); and (3) experience with unclipping the one-kidney, one-clip hypertensive rat. Unclipping after an anastomosis between the ureter and the vena cava caused the MAP to return to normal levels in an average of 20 hr. At an average of 5 hr, when the MAP had dropped an average of 34 mm Hg (from approximately 190), an exchange infusion was started and blood was collected from the renal vein. The plasma was separated, lyophilized, and extracted for total lipids. The lipids were subjected to two TLC procedures and tested for vasodepressor activity. Renal venous effluent, under those conditions, yielded a considerable amount of vasodepressor lipid that was similar to that derived from fresh renal medulla. Controls (normal, nephrectomized, and hypertensive animals) yielded little or no such lipid. Indomethacin did not interfere with the derivation of the vasodepressor lipid. As the MAP was lowered and the ANRL-like lipid appeared in the renal venous blood, the RICs degranulated. The RICs appear to be the source of the antihypertensive lipid.
当肾脏发挥其非排泄性降压功能时，ANRL是从肾静脉流出液中提取出来的。这得益于三项进展：（1）从新鲜肾髓质中提取ANRL的技术得到改进；（2）纯化后的ANRL会产生急性血管减压作用（起到血管舒张剂的作用）；（3）对单肾单夹高血压大鼠进行去夹实验的经验。在输尿管与腔静脉吻合后进行去夹，平均20小时后MAP恢复到正常水平。平均5小时时，MAP平均下降34 mmHg（从约190 mmHg开始下降），此时开始进行交换输液，并从肾静脉采集血液。分离血浆，冻干并提取总脂质。对脂质进行两次薄层层析操作，并检测其血管减压活性。在这些条件下，肾静脉流出液产生了大量与从新鲜肾髓质中提取的相似的血管减压脂质。对照组（正常、肾切除和高血压动物）产生的这种脂质很少或没有。吲哚美辛不干扰血管减压脂质的提取。随着MAP降低且肾静脉血中出现类似ANRL的脂质，肾内小动脉（RICs）脱颗粒。肾内小动脉似乎是降压脂质的来源。
