嗜热四膜虫中影响核糖体对放线菌酮抗性的突变的分离与遗传特征分析
Isolation and genetic characterization of a mutation affecting ribosomal resistance to cycloheximide in Tetrahymena.
作者信息
Ares M, Bruns P J
出版信息
Genetics. 1978 Nov;90(3):463-74. doi: 10.1093/genetics/90.3.463.
Abstract
A dominant mutation at a new locus affecting resistance to cycloheximide has been isolated by exploiting a synergistic relationship with a previously known mutation for cycloheximide resistance in Tetrahymena. The new mutation (ChxB) was induced in a line homozygous for ChxA and was recovered from that background by a new technique termed interrupted genomic exclusion. Segregation data from the interrupted genomic exclusion suggest that ChxA and ChxB are separate, linked loci showing 30% recombination. Minimal lethal doses of cycloheximide for the four possible combinations of the wild-type and mutant alleles of these two genes are: wild type 6 microgram/ml, ChxA 125 microgram/ml, ChxB 10 microgram/ml, ChxA-ChxB 175 microgram/ml.
摘要
通过利用与四膜虫中一个先前已知的抗放线菌酮突变的协同关系，在一个新位点上分离出了一个影响对放线菌酮抗性的显性突变。新突变（ChxB）是在一个对ChxA纯合的品系中诱导产生的，并通过一种称为中断基因组排除的新技术从该背景中获得。中断基因组排除的分离数据表明，ChxA和ChxB是独立的、连锁的位点，重组率为30%。这两个基因的野生型和突变等位基因的四种可能组合对放线菌酮的最小致死剂量分别为：野生型6微克/毫升，ChxA 125微克/毫升，ChxB 10微克/毫升，ChxA - ChxB 175微克/毫升。
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本文引用的文献
1
A Temperature-Sensitive Mutant in TETRAHYMENA PYRIFORMIS, Syngen 1.嗜热四膜虫，Syngen 1 中的一个温度敏感突变体。
Genetics. 1973 May;74(1):107-14. doi: 10.1093/genetics/74.1.107.
2
The Genetics of the H Serotype System in Variety 1 of Tetrahymena Pyriformis.梨形四膜虫1型中H血清型系统的遗传学
Genetics. 1960 Oct;45(10):1335-49. doi: 10.1093/genetics/45.10.1335.
3
Inheritance of T serotypes in Tetrahymena.四膜虫中T血清型的遗传
Genetics. 1967 Aug;56(4):667-81. doi: 10.1093/genetics/56.4.667.
4
Genomic exclusion: a rapid means for inducing homozygous diploid lines in Tetrahymena pyriformis, syngen 1.基因组排除：在梨形四膜虫同宗配合系1中诱导纯合二倍体系的快速方法。
Science. 1967 Feb 3;155(3762):575-7. doi: 10.1126/science.155.3762.575.
5
A cycloheximide-resistant mutant of Tetrahymena pyriformis.梨形四膜虫的一种环己酰亚胺抗性突变体。
Exp Cell Res. 1973 Oct;81(2):312-6. doi: 10.1016/0014-4827(73)90520-x.
6
Characterization of the species of the Tetrahymena pyriformis complex.梨形四膜虫复合体物种的鉴定
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7
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8
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10
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Mol Gen Genet. 1976 Dec 22;149(3):303-9. doi: 10.1007/BF00268532.
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