Mitchell S J, Bloom S R
Gut. 1978 Nov;19(11):1043-8. doi: 10.1136/gut.19.11.1043.
A specific radioimmunoassay has been developed capable of detecting 1.5 pmol VIP/l plasma with 95% confidence. The antisera employed reacted most avidly with whole VIP, partly with C terminal, but not with N terminal fragments. In 110 healthy fasting volunteers plasma VIP concentrations were estimated to lie between 0.5 and 21 pmol/l (median 1.7). No significant change was seen after ingestion of a standard test meal.
已开发出一种特异性放射免疫测定法,能够以95%的置信度检测血浆中1.5皮摩尔/升的血管活性肠肽(VIP)。所使用的抗血清与完整的VIP反应最强烈,部分与C末端反应,但不与N末端片段反应。在110名健康空腹志愿者中,血浆VIP浓度估计在0.5至21皮摩尔/升之间(中位数为1.7)。摄入标准测试餐后未见明显变化。