Bjørneklett A, Midtvedt T
Scand J Gastroenterol. 1981;16(4):473-80. doi: 10.3109/00365528109182001.
Antimicrobial drugs may affect the normal gut microflora in a potentially harmful manner. The purpose of the present study was to ascertain whether any of several tests could detect drug influence on the intestinal flora of healthy subjects. Jejunal secretions and feces were cultivated aerobically and anaerobically, with measurement of fermentation gas production in tubes supplemented with glucose or lactulose; bacterial bile acid deconjugation was measured with the 14C-GCA test; and pulmonary H2 and CH4 excretion were measured with gas-solid chromatography after lactulose and glucose ingestion in 18 healthy subjects before and after peroral treatment with either penicillin, metronidazole, or doxycyclin. Bacterial numbers and fermentation gas production were unchanged after treatment, as was the bacterial bile acid deconjugating activity. Pulmonary H2 excretion after lactulose ingestion was significantly reduced after penicillin and metronidazole but not after doxycyclin treatment. Pulmonary CH4 disappeared after metronidazole but remained unchanged after penicillin and doxycyclin treatment. It is concluded that pulmonary H2 and CH4 measurement after lactulose ingestion may serve as a sensitive determinant for drug effects on the normal gut microflora.
抗菌药物可能以潜在有害的方式影响正常肠道微生物群。本研究的目的是确定几种测试中的任何一种是否能够检测药物对健康受试者肠道菌群的影响。对空肠分泌物和粪便进行需氧和厌氧培养，在补充葡萄糖或乳果糖的试管中测量发酵气体的产生；用14C-GCA试验测量细菌胆汁酸去结合作用；在18名健康受试者口服青霉素、甲硝唑或强力霉素治疗前后，摄入乳果糖和葡萄糖后，用气固色谱法测量肺部氢气和甲烷的排泄。治疗后细菌数量和发酵气体产生没有变化，细菌胆汁酸去结合活性也是如此。摄入乳果糖后，青霉素和甲硝唑治疗后肺部氢气排泄显著减少，但强力霉素治疗后没有减少。甲硝唑治疗后肺部甲烷消失，但青霉素和强力霉素治疗后保持不变。得出的结论是，摄入乳果糖后测量肺部氢气和甲烷可作为药物对正常肠道微生物群影响的敏感指标。
