Seelig L L, Billingham R E
Transplantation. 1981 Oct;32(4):308-14. doi: 10.1097/00007890-198110000-00010.
Suspensions of viable lymph node cells (LNCs), both unlabeled and 3H-uridine labeled, from adult Fischer (FI) rats were inoculated into the lumens of established, surgically isolated segments of ileum in adult (FI x DA)F1 hybrid hosts. Vascular, lymphatic, and nerve supplies of the isolated segment were preserved. It was reasoned that subsequent hypertrophy of the draining mesenteric LN complex, on an immunogenetically specific basis (attributable to graft-versus-host (GVH) reactivity) and histological identification of labeled lymphoid cells in the intestinal wall of the isolated segment, would be indicative of the passage of inoculated cells across the intestinal epithelium. It was found that host mesenteric LNs were significantly larger on the 8th postinoculation day in animals that received 100 x 10(6) or 300 x 10(6) FI LNCs than in animals that were given similar numbers of syngeneic F1 hybrid cells (controls). This lymph node hypertrophy was dosage dependent and favorably influenced by the presence of Peyer's patches. The results of experiments involving introduction of 3H-uridine-labeled cells into isolated ileal segments and subsequent radioautography corroborated the conclusion that lymphocytes deposited in the intestinal lumen can gain access to host tissues, i.e., they are "naturally transplanted."
将成年Fischer（F1）大鼠未标记和经3H-尿苷标记的活淋巴结细胞（LNC）悬液接种到成年（F1×DA）F1杂种宿主中经手术分离的已建立的回肠段腔内。保留分离段的血管、淋巴管和神经供应。据推测，随后引流肠系膜淋巴结复合体在免疫遗传特异性基础上（归因于移植物抗宿主（GVH）反应性）肥大，以及在分离段肠壁中对标记淋巴细胞进行组织学鉴定，将表明接种的细胞穿过肠上皮。结果发现，在接种后第8天，接受100×10⁶或300×10⁶个F1 LNC的动物的宿主肠系膜淋巴结明显大于接受相同数量同基因F1杂种细胞（对照）的动物。这种淋巴结肥大是剂量依赖性的，并且派尔集合淋巴结的存在对其有有利影响。将3H-尿苷标记的细胞引入分离的回肠段并随后进行放射自显影的实验结果证实了这样的结论，即沉积在肠腔内的淋巴细胞可以进入宿主组织，即它们是“自然移植的”。
