Joel D D, Hess M W, Cottier H
J Exp Med. 1972 Apr 1;135(4):907-23. doi: 10.1084/jem.135.4.907.
Neonatal mice were given a subcapsular, intrathymic injection of thymidine-(3)H using a modified microneedle technique, and the migration of labeled cells to spleen, lymph nodes, Peyer's patches, and bone marrow was followed radioautographically with time. Assuming that nonlabeled lymphocytes migrated in the same manner as labeled lymphocytes, it can be concluded that the majority of lymphocytes present within mesenteric lymph nodes (74%) and Peyer's patches (61%), and a large proportion of those located in popliteal lymph nodes (40%) and the spleen (26%), were of thymic origin. Evidence is presented indicating that these are minimum values. The difference in the magnitude of thymic cell migration to gut-associated lymphoid tissue on the one hand and to the spleen and popliteal lymph node on the other hand was tentatively attributed to antigenic stimulation from the intestinal flora which develops during the first days of life. Thymus-derived lymphocytes were scattered throughout the lymph node cortex and splenic follicles. No noticeable thymic cell migration to the bone marrow was found. Labeling indices in the peripheral lymphoid organs paralleled those of cortical thymic lymphocytes suggesting the thymic cortex as the major source of migrants. By 2 days postinjection, the mean grain counts of labeled lymphocytes in all peripheral lymphoid tissues were higher than the mean grain counts of labeled lymphocytes in the thymus. At 7 days postinjection heavily labeled cells constituted 11-16% of the labeled population in peripheral tissues while they were absent from the thymic cortex. These results indicate that a fraction of thymus-derived cells, upon settling in the periphery, remained in, or reentered, a nonproliferative phase for at least 7 days. Conversely, many thymus-derived lymphocytes underwent division in the periphery and/or penetrated the intestinal epithelium. Since the relative number of thymus-derived cells found in the mesenteric lymph nodes of 1- and 2-day old mice was considerably higher than the percentage of cells at this site having the theta (theta) alloantigen, as reported by other authors, the possibility exists that theta-antigen on thymus-derived lymphocytes may, at least in a fraction of these cells, no longer be detectable as they reach the peripheral organs.
采用改良的微针技术给新生小鼠进行胸腺被膜下、胸腺内注射胸腺嘧啶核苷 -³H，并通过放射自显影术随时间追踪标记细胞向脾脏、淋巴结、派伊尔结和骨髓的迁移情况。假设未标记的淋巴细胞与标记的淋巴细胞迁移方式相同，可以得出结论：肠系膜淋巴结（74%）和派伊尔结（61%）中存在的大多数淋巴细胞，以及腘窝淋巴结（40%）和脾脏（26%）中很大一部分淋巴细胞，都起源于胸腺。有证据表明这些是最小值。胸腺细胞向肠道相关淋巴组织的迁移量与向脾脏和腘窝淋巴结的迁移量之间的差异，初步归因于出生后最初几天发育的肠道菌群的抗原刺激。胸腺来源的淋巴细胞散布在淋巴结皮质和脾滤泡中。未发现明显的胸腺细胞向骨髓迁移。外周淋巴器官中的标记指数与皮质胸腺淋巴细胞的标记指数平行，表明胸腺皮质是迁移细胞的主要来源。注射后2天，所有外周淋巴组织中标记淋巴细胞的平均颗粒计数高于胸腺中标记淋巴细胞的平均颗粒计数。注射后7天，外周组织中标记群体中重度标记的细胞占11 - 16%，而胸腺皮质中没有此类细胞。这些结果表明，一部分胸腺来源的细胞在外周定居后，至少7天处于非增殖期或重新进入非增殖期。相反，许多胸腺来源的淋巴细胞在外周进行分裂和/或穿透肠上皮。由于1日龄和2日龄小鼠肠系膜淋巴结中发现的胸腺来源细胞的相对数量，大大高于其他作者报道的该部位具有θ同种异体抗原的细胞百分比，因此存在这样一种可能性，即胸腺来源淋巴细胞上的θ抗原，至少在这些细胞的一部分中，当它们到达外周器官时可能不再可检测到。
