Smith J R, Whitney R G
Science. 1980 Jan 4;207(4426):82-4. doi: 10.1126/science.7350644.
At several points during the growth of a clone of human embryonic lung fibroblasts in vitro, 100 to 200 cells were removed at random and the proliferative potential of each cell was determined. At each sample point, a wide variation in remaining population doubling ability was observed among the individual cells and the distributions of doubling potentials were distinctly bimodal. Furthermore, the two cells arising from a single mitosis differed in their ability to proliferate by as many as eight population doublings (256-fold in the number of cells produced). The results suggest that a stochastic process is responsible for determining the limited proliferative potential of human embryonic lung fibroblasts.
在人胚胎肺成纤维细胞克隆体外生长的几个阶段，随机取出100到200个细胞，并测定每个细胞的增殖潜能。在每个取样点，观察到各个细胞之间剩余群体倍增能力存在很大差异，并且倍增潜能的分布明显呈双峰状。此外，由一次有丝分裂产生的两个细胞在增殖能力上相差多达八次群体倍增（产生的细胞数量相差256倍）。结果表明，一个随机过程决定了人胚胎肺成纤维细胞有限的增殖潜能。
